What is the purpose of a protein binding microarray?
Protein-binding microarrays (PBMs) are a robust and powerful HT platform for studying DNA-binding specificity of TFs. Analysis of PBM-determined DNA-binding profiles has provided new insight into the scope and mechanisms of TF binding diversity.
What is a transcription factor and how does it influence protein production?
Transcription factors are proteins involved in the process of converting, or transcribing, DNA into RNA. Transcription factors include a wide number of proteins, excluding RNA polymerase, that initiate and regulate the transcription of genes.
How do you find the transcription factor of a protein?
To qualify as a transcription factor, a protein must possess two qualities. 1) Ability to bind to DNA and 2) recruit RNA polymerase/alter transcription of a gene.
What are the different types of protein microarrays?
Three types of protein microarrays are currently used to study the biochemical activities of proteins: analytical microarrays, functional microarrays, and reverse phase microarrays.
How does a protein array work?
Protein microarrays, also known as protein chips, are miniaturized and parallel assay systems that contain small amounts of purified proteins in a high-density format (1). They allow simultaneous determination of a great variety of analytes from small amounts of samples within a single experiment.
What is DNA microarray technology?
Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. A sample containing DNA or RNA is placed in contact with the gene chip.
What is the purpose of transcription factors?
How does a transcription factor work?
Transcription factors are proteins possessing domains that bind to the DNA of promoter or enhancer regions of specific genes. They also possess a domain that interacts with RNA polymerase II or other transcription factors and consequently regulates the amount of messenger RNA (mRNA) produced by the gene.
How do protein arrays work?
Can proteins be printed as microarrays for high-throughput function determination?
MacBeath G., Schreiber S.L. Printing proteins as microarrays for high-throughput function determination. Science. 2000;289:1760–1763.
Is there a quantitative protein interaction network for the ERBB receptors?
A quantitative protein interaction network for the ErbB receptors using protein microarrays. Nature. 2006;439:168–174. [PubMed] [Google Scholar] Kattah M.G., Alemi G.R., Thibault D.L., Balboni I., Utz P.J. A new two-color Fab labeling method for autoantigen protein microarrays. Nat. Methods. 2006;3:745–751. [PubMed] [Google Scholar]
What can microarrays tell us about the binding affinities of peptides?
The microarrays were probed with eight concentrations of each peptide, and the resulting fluorescence data was used to calculate dissociation constants to determine the binding affinities of the peptide-binding domain interactions.
What is reverse phase protein microarray (RPA)?
A third type of protein microarray, related to analytical microarrays, is known as a reverse phase protein microarray (RPA). In RPA, cells are isolated from various tissues of interest and are lysed. The lysate is arrayed onto a nitrocellulose slide using a contact pin microarrayer.