What is Nonidet P-40 Substitute?
Nonidet® P40 (substitute) is a non-ionic, non-denaturing detergent suitable for solubilizing and isolation of membrane proteins. Has been used to solubilize cerebral GABA receptors. Also useful in solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors.
What is Nonidet P-40 used for?
Nonidet P40 is: Used for solubilizing membrane proteins during isolation of membrane-protein complexes. Included in buffers for microplate assays.
Is igepal the same as NP-40?
Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”. Both are octylphenoxy poly(ethyleneoxy)ethanol.
How do you dilute NP-40?
Directions: 1) Mix 20 ml of NP-40 with 80 ml of ddH2O by stirring. 2) Store at 4°C. Note: Nonidet P-40 is chemically identical to Igepal-CA-630.
How do you make a RIPA lysis buffer?
Preparation of RIPA lysis buffer: • 10mM Tris-HCl, pH 8.0 • 1mM EDTA • 0.5mM EGTA • 1% Triton X-100 • 0.1% Sodium Deoxycholate • 0.1% SDS • 140mM NaCl • Dilute with dH2O • This solution is stable at room temperature. Add 1mM PMSF immediately before use.
What is RIPA buffer used for?
RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
What is igepal used for?
General description. IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.
What is the function of the NP-40 detergent in the extraction buffer?
NP-40 lysis buffer The solubilizing agent is NP-40, which can be replaced by other detergents at different concentrations. Since NP-40 is a nonionic detergent, this lysis buffer has a milder effect than RIPA buffer. It can be used when protein functions are to be retained with minimal disruption.
What is NP-40 lysis buffer?
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions. Solubility. It is soluble in water.
Does NP-40 lysis nuclei?
NP-40 and Triton X-100 will not lyse nuclear membranes. After lysis, pellet the nuclei by centrifugation and transfer the supernatant to a new tube. If you wish to isolate both the nuclear and soluble fractions, resuspend the nuclear pellet in RIPA buffer. NP-40 is also marketed under the name Igepal CA-630.
How do I make a NP-40 lysis buffer?
The Unfolded Protein Response and Cellular Stress, Part A Prepare 1% NP40 lysis buffer (LB). (To make 50 ml of 1% NP40 lysis buffer, add 5 ml of 10% NP40, 500 μl of 1 mM EDTA, 1.5 ml of 150 mM NaCl, and 1 ml of 20 mM Tris–Cl, pH 7.4; and bring the total volume to 50 ml using dH2O.)
What is the function of NP-40 detergent?
NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability.