What is c33a cell line?
Description. The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies. The line exhibited a hypodiploid karyotype initially, and an epithelial morphology. Karyological instability was observed with continued passage.
What are SiHa cells?
SiHa is a cell line isolated from fragments of a primary uterine tissue sample from a 55-year-old, female, Japanese patient with squamous cell carcinoma. Genes expressed are p53+ and pRB+. This cell line is a suitable transfection host.
What are cancerous cell lines?
(KAN-ser sel line) Cancer cells that keep dividing and growing over time, under certain conditions in a laboratory. Cancer cell lines are used in research to study the biology of cancer and to test cancer treatments.
What are derived cell lines?
In cancer research, collections of tumor-derived cell lines are often used as models because they carry hundreds to thousands of aberrations that arose in the tumor from which they were derived. Cancer cell lines are used to study many biologic processes and have been widely used in pharmacogenomics studies.
What is the doubling time of HeLa cells?
HeLa cells growing in suspension have a doubling time of 23 hours and the maximum cell densities is approximate 3-4.
How can a cell line be obtained from a cultured cell?
Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture.
What are cell line models?
Cell lines are in vitro model systems that are widely used in different fields of medical research, especially basic cancer research and drug discovery. Their usefulness is primarily linked to their ability to provide an indefinite source of biological material for experimental purposes.
Why HeLa cells are so important?
In 1952, HeLa cells became the first human cell line that could grow and divide endlessly in a laboratory, leading scientists to label these cells “immortal”. The immortality of HeLa cells contributed to their adoption across the world as the human cell line of choice for biomedical research.
What is a PDX line?
PDX-derived cell lines from Creative Bioarray are all early passage (<10) and maintain essential histopathological features and genetic profiles of the original patient tumors including biochemical signaling, genomic mutational status, and response to tumor cell autonomously targeted therapeutics.
How many times can a HeLa cell divide?
When grown in a laboratory setting, PCD generally occurs after about 50 cell divisions. But that’s what sets HeLa apart. Under the right conditions, HeLa cells form an immortal cell line; they divide indefinitely.
How do you count HeLa cells?
Count the Hela cell with a INCYTO™ C-Chip™ disposable hemocytometer. Then dilute the HeLa cell with DMEM by serial dilution within a range of several thousand to several million cells per mL. Turn-on the Photopette® device and connect to the Photopette® iOS/Android app. Select ‘Cell counting’ as the measurement type.