How do you make glycerol 80% glycerol stock?

How do you make glycerol 80% glycerol stock?

Bacterial Glycerol Stocks

  1. Put 0.5ml bacterial culture in a sterile eppendorf tube.
  2. Add 0.5ml of sterile 80% (v/v) glycerol soution.
  3. Freeze on dry ice or directly into –70oc .
  4. Store at –70oC. Cells are best for about 4-6 months, but will probably work ok for a whole year.

How do you make 40% glycerol?

Measure out 40ml of 100% glycerol solution into a 250ml bottle. When pipetting glycerol, use ethanolsterilised scissors to cut the end off of a pipette to make pipetting easier 2. Add 60ml ddH​2​ O to the bottle.

How do I make a 50% glycerol solution?

Dilute pure glycerol in distilled water to create a 50% glycerol solution. Use a sterile pipette to measure out 10 mL of both liquids and combine them in a single flask. Stir or shake the flask thoroughly until the liquids are evenly mixed.

How do you make a 20% glycerol solution?

I prepared 80 ml of MRS broth by adding 4.176g of MRS broth to 80 ml distilled water. Then I added 20 ml of glycerol, so the amount is 100 ml.

Why do we prepare glycerol stock?

However, if you want to store bacteria for a longer time, you will need to establish glycerol stocks. The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years.

Is glycerine and glycerol the same?

Glycerol (/ˈɡlɪsərɒl/), also called glycerine in British English and glycerin in American English, is a simple polyol compound. It is a colorless, odorless, viscous liquid that is sweet-tasting and non-toxic. The glycerol backbone is found in lipids known as glycerides.

How do you make a 30% glycerol solution?

Prepare a solution of 30% glycerol (v/v) by mixing 30 ml of glycerol with 70 ml of water. Transfer the solution to a screw cap glass bottle and sterilize by autoclaving at 121°C for 15 min. Loosen the cap during autoclaving.

Can we autoclave 100% glycerol?

Yes, I have sterilised glycerol in the autoclave in a glassware. It works fine!

Can you autoclave 100% glycerol?

How do you make a 10% glycerol solution?

To prepare a 10% (v/v) solution: Dilute 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a pre-rinsed 0.22-μm filter. Store in 200-mL aliquots at 4°C.

How do you make a 5% glycerol solution?

Then there is %v/v. So a 5% glycerol solution contains 5ml glycerol in 95ml water (total volume is 100ml). Use w/v when the solutes are solid and vol/vol when they are liquid.

How do you prepare 10 glycerol?