Can Pseudomonas aeruginosa be detected by PCR?

Can Pseudomonas aeruginosa be detected by PCR?

This PCR assay was tested with 182 isolates of P. aeruginosa and 20 isolates of other bacterial species, and demonstrated 100% specificity and sensitivity. The test was also able to detect P. aeruginosa directly in clinical samples such as sputum or throat swabs obtained from cystic fibrosis patients.

What bacteria is Pseudomonas?

Pseudomonas aeruginosa is a Gram-negative, rod-shaped, asporogenous, and monoflagellated bacterium. It has a pearlescent appearance and grape-like or tortilla-like odour.

How do you test for Pseudomonas bacteria?

No serologic tests are available. Diagnosis of melioidosis can be confirmed by isolating B pseudomallei from the blood, urine, sputum, or skin lesions. Detecting antibodies to the bacteria in the blood is another method.

What is the meaning of Pseudomonas infection?

Pseudomonas infections are infections caused by a kind of bacteria called Pseudomonas that’s commonly found in soil, water, and plants. The type that typically causes infections in people is called Pseudomonas aeruginosa.

How is Pseudomonas aeruginosa detected?

Detection of P. aeruginosa colonisation is normally achieved by culture of wound swabbing on to artificial media. Typical isolation media for wound infections include blood agar and chocolate agar as well as selective agars such as Mac- Conkey agar and cetrimide-based media.

How do you get Pseudomonas bacteria?


  1. Inoculate P. aeruginosa into LB broth with appropriate antibiotics and grow culture of P.
  2. Add 0.6 ml of overnight culture to cryogenic vial with 0.4 ml of 50% glycerol.
  3. Mix solution well by vortexing on a medium setting or by repeated inversions.
  4. Store bacterial glycerol stock in −80°C freezer.

How do I know if I have Pseudomonas stutzeri?

Characterization. Pseudomonas stutzeri is a Gram-negative, rod-shaped, non-spore-forming bacterium that is typically 1–3 microns long and 0.5–0.8 microns wide. It is a microbe that tests positive for both the catalase and oxidase tests.

How do you get rid of Pseudomonas bacteria?

If you have a Pseudomonas infection, it can usually be treated effectively with antibiotics. But sometimes the infection can be difficult to clear completely. This is because many standard antibiotics don’t work on Pseudomonas. The only type of tablet that works is ciprofloxacin.

Can a blood test detect Pseudomonas?

The Carba NP test has been evaluated to detect carbapenemase-producing Pseudomonas spp. directly from blood cultures. This rapid and cost-effective test permits an early identification of carbapenemase-producing Pseudomonas spp. directly from blood cultures with excellent sensitivity and specificity.

What diseases does Pseudomonas aeruginosa cause?

Pseudomonas aeruginosa causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in patients with severe burns and in cancer and AIDS patients who are …