Are zinc fingers transcription factors?
Zinc finger proteins are the largest transcription factor family in human genome. The diverse combinations and functions of zinc finger motifs make zinc finger proteins versatile in biological processes, including development, differentiation, metabolism and autophagy.
What is a homeodomain transcription factor?
Homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. These family members are characterized by a helix-turn-helix DNA-binding motif known as the homeodomain (encoded by the 180 bp homeobox sequence element).
How does zinc finger nuclease work?
Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes.
What are Znf genes?
The C2H2, or Krüppel-type zinc finger (ZNF), is the most common DNA-binding motif found in eukaryotic TF proteins; ZNF proteins typically contain multiple C2H2 motifs joined together in tandem arrays. Proteins of this type are ancient and numerous, encoded by large and diverse gene families in all eukaryotic genomes.
Are zinc fingers dimers?
The C2H2 zinc finger is the most prevalent protein motif in the mammalian proteome. Two C2H2 fingers in Ikaros are dedicated to homotypic interactions between family members. We show here that these fingers comprise a bona fide dimerization domain.
What is a zinc finger?
A zinc finger is a finger-shaped protein fold that is formed by specific amino acids in the protein binding to a zinc ion. These proteins often bind to DNA and RNA because their shape allows close interaction of the domain with the nucleotides of DNA and RNA.
What is zinc finger protein (ZFP)?
Zinc finger protein (ZFP) is a common DNA binding domain found in many transcription factors. It consists of ∼30 amino acids that may recognize three base pairs of DNA.
How does the zinc finger bind to DNA?
The zinc finger binds to DNA, and through the interaction of amino acids at its periphery with base pairs at the center of the DNA double helix. It is a compact protein domain, and its small size allows it to have close proximity with DNA base pairs.
What are the DNA-binding properties of Myeloid zinc finger protein MZF1?
Characterization of the DNA-binding properties of the myeloid zinc finger protein MZF1: two independent DNA-binding domains recognize two DNA consensus sequences with a common G-rich core. NCBI Skip to main content Skip to navigation Resources How To About NCBI Accesskeys